Journal: Materials Today Bio

Article Title: Bioproduction of ∼10 knt single-stranded DNA for constructing large DNA origami structures

doi: 10.1016/j.mtbio.2026.103092

Figure Lengend Snippet: Schematic illustration of the biosynthesis of long ssDNA. The PCR-amplified dsDNA fragments were first assembled into recombinant phagemids using Gibson assembly and subsequently transformed into E. coli for amplification. This process was carried out in both shake-flask cultures and bioreactor systems, with systematic optimization of cultivation conditions to markedly enhance phage particle production. Finally, the resulting ssDNA was harvested and purified, and subsequently employed as a scaffold strand for the assembly of large-scale DNA origami nanostructures.

Article Snippet: Milligram-scale production of synthetic phage particles was carried out in a stirred-tank bioreactor (TMAXTREE Tmax Bio-3L) with a working volume of 1 L. XL1-Blue cells harboring the correctly assembled phagemid were grown to an OD 600 of 0.5, and 4 mL of this culture was inoculated into 2 × YT medium supplemented with 100 μg/mL ampicillin.

Techniques: Amplification, Recombinant, Transformation Assay, Purification

Journal: Materials Today Bio

Article Title: Bioproduction of ∼10 knt single-stranded DNA for constructing large DNA origami structures

doi: 10.1016/j.mtbio.2026.103092

Figure Lengend Snippet: Bioreactor production of 10,563 nt ssDNA. (a) Schematic illustration of ssDNA biosynthesis in a bioreactor system. (b) Growth curve of E. coli during bioreactor cultivation, showing cell density as a function of cultivation time. (c) Effect of bioreactor cultivation time on ssDNA yield, showing a maximum ssDNA production at 30 h of cultivation. All data are presented as mean ± SD (n = 3 biological replicates). Error bars represent standard deviations. (d) Comparison of ssDNA yields obtained using the bioreactor-based approach and reported methods .

Article Snippet: Milligram-scale production of synthetic phage particles was carried out in a stirred-tank bioreactor (TMAXTREE Tmax Bio-3L) with a working volume of 1 L. XL1-Blue cells harboring the correctly assembled phagemid were grown to an OD 600 of 0.5, and 4 mL of this culture was inoculated into 2 × YT medium supplemented with 100 μg/mL ampicillin.

Techniques: Comparison